primary human retinal microvascular endothelial cells hrmecs  (Innoprot Inc)

Journal: Communications Biology

Article Title: Combination of apolipoprotein-A-I/apolipoprotein-A-I binding protein and anti-VEGF treatment overcomes anti-VEGF resistance in choroidal neovascularization in mice

doi: 10.1038/s42003-020-1113-z

Figure Lengend Snippet: a Effect of AIBP and HDL 3 on lipid rafts of HRMECs. Cells were stained for lipid rafts (green, D4-EGFP) and nuclei (blue, DAPI) after preincubation with control media, AIBP, HDL 3 , or AIBP plus HDL 3 . b Quantification of the area of lipid rafts per cell. N = 92, 54, 70, and 86 cells for control, AIBP, HDL 3 , and AIBP + HDL 3 , respectively. c Effect of AIBP and HDL 3 on endothelial cell tube formation using the Matrigel-based assay. The total segment length of HRMEC tubes was quantified after various treatments. N = 12, 9, 6, 6, and 6 for control, AIBP, HDL 3 , AIBP + HDL 3 , and MβCD, respectively. Representative images ( d ) and quantification of microvascular sprouting area ( e ) after AIBP treatment. N = 3 explants per group. Data represent mean ± SEM in b , c , e . Statistical analysis was performed by one-way ANOVA with Tukey post hoc analysis ( b , c ) or two-tailed Student’s t -test ( e ).

Article Snippet: Primary human retinal microvascular endothelial cells (HRMECs) were purchased from Cell Systems Corporation (Kirkland, WA, USA).

Techniques: Staining, Control, Matrigel Assay, Two Tailed Test

Journal: Communications Biology

Article Title: Combination of apolipoprotein-A-I/apolipoprotein-A-I binding protein and anti-VEGF treatment overcomes anti-VEGF resistance in choroidal neovascularization in mice

doi: 10.1038/s42003-020-1113-z

Figure Lengend Snippet: a Peritoneal macrophage isolated from 1-month, 8-month, and 18-month 57Bl6/J mice were treated with AIBP and apoA-I, and stained with oil red O. b Quantification on the number of lipid droplets per cell in macrophages. N = 25 (control) and 28 (AIBP/apoA-I) for 8-month mice, 43 (control) and 39 (AIBP/apoA-I) for 18-month mice. c Effect of AIBP on old macrophages’ ability to promote angiogenesis of HRMECs. Macrophages (MΦ) isolated from 1-month and 18-month mice were pretreated with different combination of AIBP and apoA-I and co-cultured with HRMECs. d Quantitative analysis of total segment length of tubes of HRMECs in c . N = 7 (without co-culture), 10 (1-month MΦ + control), 6 (1-month MΦ + AIBP/apoA-I), 14 (18-month MΦ + control), and 13 (18-month MΦ + AIBP/apoA-I) fields. Data represent mean ± SEM in b , d . Statistical analysis was performed by two-tailed Student’s t -test ( b ) or one-way ANOVA with Tukey post hoc analysis ( d ). Scale bar, 25 µm in a , 1000 µm in c .

Article Snippet: Primary human retinal microvascular endothelial cells (HRMECs) were purchased from Cell Systems Corporation (Kirkland, WA, USA).

Techniques: Isolation, Staining, Control, Cell Culture, Co-Culture Assay, Two Tailed Test

Journal: Communications Biology

Article Title: Combination of apolipoprotein-A-I/apolipoprotein-A-I binding protein and anti-VEGF treatment overcomes anti-VEGF resistance in choroidal neovascularization in mice

doi: 10.1038/s42003-020-1113-z

Figure Lengend Snippet: a Representative images and quantification of microvascular sprouting area from Naxe −/− and WT adult mouse choroid explants. N = 3 choroid explants for WT , 4 for Naxe −/− mice. b Representative images of CNV lesions labeled by Alexa 568-isolectin on RPE-choroid flatmounts and quantification of CNV areas from Naxe −/− and WT adult mice. N = 33 laser spots per group. c Representative images of CNV lesions and quantification of CNV areas after intravitreal injection of a rabbit anti-AIBP antibody or a control IgG. N = 28 laser spots per group. Scale bar is 20 µm in b and c . Data represent mean ± SEM in a – c . Statistical analysis was performed by two-tailed Student’s t -test.

Article Snippet: Primary human retinal microvascular endothelial cells (HRMECs) were purchased from Cell Systems Corporation (Kirkland, WA, USA).

Techniques: Labeling, Injection, Control, Two Tailed Test